UJI TOKSISITAS
EKSTRAK ETANOL Sonneratia alba
METODE BRINE SHRIMP LETHALITY TEST (BSLT) PADA Artemia salina
1) Fakultas Perikanan
dan Ilmu Kelautan
Universitas Borneo
Tarakan
Sonneratia alba can
bear fruit all the time on the coast of North Borneo and especially the city of
Tarakan. This coastal mangrove plant has not seasoning fruitfully that could
potentially be used but until now not been optimal utilization fruit in
nutrient enrichment on Artemia salina
as natural feed (bioenrichment) for
fry tiger shrimp (Penaeus monodon).This
study aims to determine the toxicity of ethanol extract of Sonneratia alba through the method of
brine shrimp lethality test (BSLT)
which uses a nauplii Artemia salina
ethanol extract Sonneratia alba
is known to have an active compound content in the form of alkaloids,
flavonoids, triterpenoids, carbohydrates, carotenoids, tannins, and coumarin.
Also, based on the test on selective media TCBSA (Thiosulphate Citrate Bile Salt Sucrose Agar) through a paper
disc diffusion technique on the growth of bacteria Vibrio harveyii capable of producing inhibitory characterized by
the formation of a clear zone diameter of 2.50 ± 0.31 cm at a concentration of
2% , BSLT testing to obtain the LC50 value of the ethanol extract S.alba of the survival of nauplii A. salina using ethanol extract concentration difference S.alba as a treatment that is 0 ppm,
10 ppm, 15 ppm, 20 ppm and 25 ppm. Test bottles that had contained the ethanol
extract test concentrations S.alba,nauplii
A.salina, and solvent sea water
is stored in a fairly light and oxygen for 24 hours; After 24 hours, counted
the number of nauplii A.salina dead.
At the ethanol extract treatment S.alba
BSLT testing shows the results of LC50 of 10.2 ppm (10 ppm optimal
treatment) with a value of probit R2 = 0.84 expressed by regression
Y = 2.7741 X + 2.2022.
Keywords: Soneratia alba, Artemia salina, toxicity, BSLT
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