ETANOL EXTRACTION OF Sonneratia alba’s
FRUIT FOR INHIBITING Vibrio harveyii IN VITRO
Gloria Ika Satriani,
Jimmy Cahyadi, Ery Gusman,
Eka
Nur Juliana 1)
Pedada or Sonneratia sp. mangrove plant is commonly found on the A2 zone which has submerged tidal area 10-19 times per month, the area closest to the sea, and grow
on muddy substrate in rich organic matter with the root system of this vegetation has formed by crabbed (pneumatofora for breathing). Sonneratia types most common in coastal areas Tarakan Island is S. alba and S. caseolaris which has similar but differentiated by coloring of flowers petals where are white flowers to S.alba and red to S.caseolaris. S.alba is being
fruitfull all the time and not depend of season, so it has the potential to be exploited but until
now not yet optimal utilization of this fruit. This study aims to utilize of fruit extract S.alba
as an antibacterial for vibriosis disease in black tiger shrimp fry (P.monodon).
The extraction was done in FPIK UBT Environment Laboratory with 100% ethanol (pa)
which comparison
of simplisia:
ethanol = 1:
2 during the following hours filtrate 3x24 maceration result
processed by used the rotary evaporator temperature 40-50◦C during 1,5-2 hours. Crude drug used in this study
a whole fruit S.alba (rind, flesh, and seeds). Concentrating the extraction
is
done by oven at a temperature of 60◦C to obtain a thick paste extract S.alba. The test results
showed a
thick paste phytochemical extracts S.alba 100% ethanol solvent contains of alkaloids, flavonoids, triterpenoids,
carbohydrates,
carotenoids,
coumarin,
and tannins. The
results of
antibacterial tests showed Vibrio harveyii extract inhibited at all concentrations S.alba ethanol.
Keywords: S.alba, V.harveyii,
phytochemicals
https://drive.google.com/open?id=1dOpQlup7XTvMx-vNuaszeeFVVyWkcTvf
https://drive.google.com/open?id=1dOpQlup7XTvMx-vNuaszeeFVVyWkcTvf
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